科研動態(tài)

近年來水產(chǎn)養(yǎng)殖中的常用藥物恩諾沙星在鯽中的殘留問題比較突出，但目前國家未對其在鯽中的休藥期作出明確規(guī)定。為做到鯽養(yǎng)殖中科學(xué)合理使用恩諾沙星，在12~15 ℃水溫條件下，以60 mg/kg.b.w.劑量，對體質(zhì)量為（250±10）g的健康方正銀鯽（Carassius auratus gibelio）灌服恩諾沙星；在灌服后0~120 d內(nèi)不間斷采樣，用高效液相色譜-串聯(lián)質(zhì)譜儀檢測，研究恩諾沙星及其代謝產(chǎn)物環(huán)丙沙星在方正銀鯽鰓、血漿、肌肉、皮膚、肝臟和腎臟中的代謝及消除規(guī)律，從而為恩諾沙星在方正銀鯽中的休藥期制定提供參考。結(jié)果顯示：灌藥后，恩諾沙星在方正銀鯽血漿、肌肉、肝臟、鰓、腎臟和皮膚中的達峰時間tmax分別為9、18、24、24、48和48 h，腎臟、肝臟、肌肉、血漿、鰓和皮膚中的達峰濃度cmax分別為30.490、21.372、18.715、16.636、15.157和11.663mg/kg；皮膚中的消除半衰期t1/2β最大，為338.2 h，血漿中最小，為93.303 h；代謝產(chǎn)物環(huán)丙沙星的代謝及消除趨勢與恩諾沙星大致相同，血漿中達峰值產(chǎn)生最早，tmax為18 h，腎臟中的峰濃度最大，cmax為547.26 μg/kg。結(jié)果表明，方正銀鯽以單次口灌60 mg/kg.b.w.劑量恩諾沙星，皮膚和肌肉中的恩諾沙星和環(huán)丙沙星總量需要1200度日才能滿足限量要求。本研究為恩諾沙星在鯽魚養(yǎng)殖中的科學(xué)使用提供了數(shù)據(jù)支撐。

Establishmentof a Real-time RPA Method for Rapid Detection of Lymphocytic ChoriomeningitisVirus

Inrecent years，the residues of Enrofloxacin that has been commonly used inaquaculture have become more serious in Carassius auratus gibelio，however，no any clear regulation on itswithdrawal period has been developed by the state. In order to scientificallyuse the Enrofloxacin in carp breeding，the followingexperiment was carried out. Healthy Carassius auratus gibelio with body mass of（250±10）g was fedwith Enrofloxacin at a dose of 60 mg/kg.b.w. under the water temperature of 12to 15 ℃，and then incessantly sampled within 0 to 120 dto test by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS），hence the metabolism mechanism wasstudied，as well as the elimination rule of Enrofloxacinand its metabolite（Ciprofloxacin）in gill，plasma，muscle，skin，liver and kidney，which could provide some references for defining its withdrawalperiod. The results showed that，after administration，the peak time（tmax）ofEnrofloxacin in plasma，muscle，liver，gill，kidney and skin was 9，18，24，24，48 and 48 h，respectively and the peakconcentration（cmax）was 16.636，18.715，21.372，15.157，30.490 and 11.663 mg/kg，respectively. Theelimination half-life（t1/2β）inskin was the maximum，which was 338.2 h，and in plasma it was the minimum（93.303 h）. The metabolism and elimination trend of Ciprofloxacin was similarto that of Enrofloxacin，specially，the peak value first appeared in plasma with tmax of 18 h，and the peak concentration reached the maximum in kidney with cmaxof 547.26 μg/kg. Therefore，aconclusion was given that it would take more than 1 200 degree-days for thetotal amount of Enrofloxacin and Ciprofloxacin in skin and muscle to reach therequired limit if Carassius auratus gibelio was orally administered withEnrofloxacin at a dose of 60 mg/kg.b.w each time. Therefore，some data supports were provided by this study for scientific use ofEnrofloxacin in carp breeding.

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