科研動(dòng)態(tài)

云南省香格里拉市某藏豬場(chǎng)育肥豬出欄屠宰時(shí)，陸續(xù)發(fā)現(xiàn)部分個(gè)體小腸腸系膜漿膜下有大量葡萄串狀透明無(wú)味氣泡，病因不明，但病豬在仔豬階段有腹瀉病史。為調(diào)查發(fā)病原因和致病機(jī)理，以便提出防控措施，采集該養(yǎng)豬場(chǎng)4頭患病豬和1頭正常豬的抗凝全血、小腸組織和腸系膜淋巴結(jié)，進(jìn)行病毒核酸PCR擴(kuò)增、細(xì)菌分離培養(yǎng)和組織病理顯微觀察。在患病豬中，未檢出與腹瀉有關(guān)的豬瘟病毒、豬流行性腹瀉病毒、傳染性胃腸炎病毒和輪狀病毒核酸；血瓊脂有氧和厭氧培養(yǎng)均只分離到大腸桿菌，并排除常見(jiàn)致病血清型及常見(jiàn)毒力基因；組織病理檢查發(fā)現(xiàn)，腸黏膜下層有大量空泡，腸腺及其杯狀細(xì)胞體積明顯增大，組織間隙擴(kuò)大，腸系膜及其淋巴結(jié)分別見(jiàn)嗜酸性粒細(xì)胞和巨噬細(xì)胞增多，病變腸系膜淤血。分析認(rèn)為，該病病因應(yīng)考慮養(yǎng)殖區(qū)域海拔較高，豬腸內(nèi)大腸桿菌異常發(fā)酵產(chǎn)氣的可能，但如何防治需進(jìn)一步研究。

Histopathological Observation and Pathogen Detection of

Porcine Intestine Pneumatosis Cystoides in Shangri-La City of Yunnan Province

A large number of grape-shaped transparent odorless bubbles were successively found under serosa of small intestine mesenterium in some fattening pigs in a Tibetan pig farm in Shangri-La City of Yunnan Province when the pigs were sold for slaughtering，with unknown causes，while the sick pigs had suffered from diarrhea at the piglet stage. In order to investigate the cause and pathogenesis so as to put forward prevention and control measures，the anticoagulant whole blood，small intestine tissues and mesenteric lymph nodes were collected from four sick pigs and one normal pig for PCR amplification of viral nucleic acids，bacterial isolation and culture and microscopic observation of histopathology. No any classical swine fever virus，porcine epidemic diarrhea virus，transmissible gastroenteritis virus and rotavirus nucleic acid related to diarrhea were detected；only Escherichia coli was isolated by blood agar aerobic and anaerobic culture，and the common pathogenic serotypes and virulence genes were excluded；the following symptoms were found through pathological examination such as a large number of vacuoles in the intestinal submucosa，the increased volume of intestinal gland and its goblet cells，the widened space among various tissues，the increased eosinophils and macrophages in the mesentery and its lymph nodes，and congestion in the diseased mesentery. It was concluded that the disease was likely to be caused by higher altitude，abnormal fermentation and air produced by E. coli，and it was necessary to continue to study how to prevent and control it.

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