科研動態(tài)

為深入了解嚴重急性呼吸綜合征冠狀病毒2型（SARS-CoV-2）S、M、E、N蛋白的分子生物學特征和基本結(jié)構(gòu)特征，選取S、M、E、N蛋白序列進行生物信息學分析和預(yù)測，使用DNAstar、SignalP、TMHHM、PSORT Prediction、BUSCA和ABCpred軟件，分析并相互驗證4種蛋白的結(jié)構(gòu)域特征和S蛋白的潛在B細胞抗原表位，結(jié)果預(yù)測出了4種結(jié)構(gòu)蛋白的功能結(jié)構(gòu)域，并分析預(yù)測出S蛋白潛在的B細胞抗原表位為25~29、75~81、112~116、148~152、773~779 aa。研究結(jié)果可為SARS-CoV-2相關(guān)的分子生物學和結(jié)構(gòu)生物學研究提供參考，并為疫苗開發(fā)等提供理論依據(jù)。

Characteristics of Four Kinds of Structural Proteins of SARS-CoV-2

In order to further identify the molecular biological characteristics and general structural characteristics of S，M，E and N proteins of severe acute respiratory syndrome coronavirus type 2（SARS-CoV-2），the amino acid sequences of the above proteins were selected for bioinformatics analysis and prediction. The structural domain characteristics of the above proteins and the potential B cell epitopes of S protein were analyzed and mutually verified by DNAstar，SignalP，TMHHM，PSORT Prediction，BUSCA and ABCpred softwares. The results showed that the functional domains of the four proteins were predicted，and the potential B cell epitopes of S protein was analyzed and predicted to be 25 to 29，75 to 81，112 to 116，148 to152 and 773 to 779 aa. Based on the results，some references were provided for further study on molecular biology and structural biology related to SARS-CoV-2，and theoretical basis was provided for vaccine development.

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